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CH01b12
Primer features
Name Sequence Length Tm  
CH01b12.F CGCATGCTGACATGTTGAAT 20 nt 56.4 °C Primer Stats
CH01b12.R CGGTGAGCCCTCTTATGTGA 20 nt 60.5 °C
SSR info
SSR repeat type Perfect
Locus type multi-locus
Detected loci
Alleles size range 125-178
Number of alleles detected 9
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 150
Linkage group(s)
Developed by ETH Zuerich
Other maps Braeburn-8 Discovery-12 Discovery-13 Fiesta-4 Fiesta-13 Fiesta x Totem-13 Apple Integrated Map-4 Apple Integrated Map-12 Apple Integrated Map-13 M.27 x M.116-4 M.27 x M.116-12 M.27 x M.116-13
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01b12 ttcgagttgtgccgcatgctgacatgttgaatctctttaaaacttcntcaatgtaacttctttgtgacaaccgtaataat cccttctctctctctctctctctctctctctctctctctctctcttttaatctcgatggngatcacataagagggctcac cgacttggtatcatctaaaaaaatcatactatttgtnggcaataatatgtcatcaacattgagtaccaaaataatgaagt tgctcccactgactttcaagtaaatgcanacatcatangggttttccacanngccatgatccttaacaacacgatgggaa tt
User's notes
Luca Gianfranceschi (luca.gianfranceschi@unimi.it)
Date 7 October 2005 - Time: 16:07
New features have been added to the web pages.
Please pay attention to the difference between Locus and Marker type.


Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
127:128:178
125:127:160:178
126:128:152:nul

126:128
127:128:178
125:127:178
126:127:178


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