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CH01e12
Primer features
Name Sequence Length Tm  
CH01e12.F AAACTGAAGCCATGAGGGC 19 nt 57.3 °C Primer Stats
CH01e12.R TTCCAATTCACATGAGGCTG 20 nt 56.4 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 246-278
Number of alleles detected 5
Expected heterozygosity 0.67
PCR annealing temp 60
Sequenced allele size 276
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-8 Prima-8 Braeburn-11 Telamon-11 Fiesta x Totem-8 Fiesta x Totem-15 Apple Integrated Map-8 M.27 x M.116-8
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01e12 gctcattcgacacgtggactaaaactgaagccatgagggcatgcacgcacggtatataatcaagacattcttatcttcct cctcttaaaccagacacattcctctctctctctctctctctctctctctctctctctctctctctctctctctctctctc tgtgcaagattgtgagtgagaagcggagagatcgatgatggggaaaaggaggagtatattgctcttgttagtaatgggat tgtttgtttttagtgttggagcagcagatcatcagcctcatgtgaattggaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
246:250
250
250

262:278
250:278
250:278
250


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