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CH01f02
Primer features
Name Sequence Length Tm  
CH01f02.F ACCACATTAGAGCAGTTGAGG 21 nt 59.4 °C Primer Stats
CH01f02.R CTGGTTTGTTTTCCTCCAGC 20 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 174-206
Number of alleles detected 7
Expected heterozygosity 0.79
PCR annealing temp 60
Sequenced allele size 185
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-12 Fiesta x Discovery-12 Braeburn-10 Telamon-10 Discovery-12 Fiesta-12 Fiesta x Totem-12 Robusta5-12 Apple Integrated Map-12 M.27 x M.116-12
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01f02 tacatccaaatcttcaaccacattagagcagttgaggatgattaagtcactaaaccaaacatcctttatagtagaacaca gagagagagagagagagagagagagagagagagagagagagagtacatatttcacagagaaactaaaganggtgcataca tgtgagtactatgtttggggggctggaggaaaacaaaccagctgtgtccacaaccatctgctgctaccctatttccttcc tatattatctgcaaaacatgatacacaanggtcacacaaataaaactattagagcaacattgatcaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
180:206
182:204
184:206

180:206
184:206
180:206
180:184


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