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CH02a08
Primer features
Name Sequence Length Tm  
CH02a08.F GAGGAGCTGAAGCAGCAGAG 20 nt 62.5 °C Primer Stats
CH02a08.R ATGCCAACAAAAGCATAGCC 20 nt 56.4 °C
SSR info
SSR repeat type Perfect
Locus type multi-locus
Detected loci
Alleles size range 133-177
Number of alleles detected 15
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 152
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-10 Discovery-5 Fiesta-10 Discovery x TN10-8-10 Discovery-5 Discovery-10 Fiesta-5 Fiesta-10 Fiesta x Totem-10 Malling9-10 Apple Integrated Map-5 Apple Integrated Map-10 M.27 x M.116-5 M.27 x M.116-10
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02a08a aattatggagaagatgcaacttgggggaccctcaatgcgcttgttagaggtgtgcttatttacaagatgtacaaactaga gtcatgtaactatcttataaacatcatcttgatgtgtttaatatgtcctgcagctgctggaggagctgaagcagcagagc tctggcggcgaactccatctcaatgatgttagtatattgctctctctctctctctctctctctctctctctctctctctc tctcaatacaaacacagattggcatgctgatggctatgcttttgttggcatttcanctgaggactgcaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
139:147:151:173
145:155:165:177
139:143:151:173

153:165:167
137:147:151
137:139:147:151
137:139:151:159


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