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CH02a10
Primer features
Name Sequence Length Tm  
CH02a10.F ATGCCAATGCATGAGACAAA 20 nt 54.3 °C Primer Stats
CH02a10.R ACACGCAGCTGAAACACTTG 20 nt 58.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 143-177
Number of alleles detected 6
Expected heterozygosity 0.8
PCR annealing temp 60
Sequenced allele size 155
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta-10 Spur-Fuji x Telamon-10 Discovery-10 Fiesta-10 Mondial Gala-10 Fiesta x Totem-10 M.27 x M.116-10
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02a10 aattgtaggacacagggacctccaaactccgcggggaaatggaaagatgccaatgcatgagacaaaacggcattgacagc cttcacgttgtattcaagctcccttcacccacccaccacttgtacagtacgtaccctctctctctctctccctctctctc tctctctctctctctctctctctcatgcgtgaaacgagaccaagtgtttcagctgcgtgtctgtcccatcaatgtgttcc ttgtggttttgcaggcaggctagggtttaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
149:155
143:177
143:151

145:177
155:177
149:177
149:155


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