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CH02c06
Primer features
Name Sequence Length Tm  
CH02c06.F TGACGAAATCCACTACTAATGCA 23 nt 59.3 °C Primer Stats
CH02c06.R GATTGCGCGCTTTTTAACAT 20 nt 54.3 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 216-254
Number of alleles detected 8
Expected heterozygosity 0.85
PCR annealing temp 60
Sequenced allele size 249
Linkage group(s)
Developed by ETH Zuerich
Other maps A722-7 x Golden Delicious-2 Discovery-2 Discovery-9 Discovery x TN10-8-2 Fiesta-2 Braeburn-6 Telamon-6 Discovery-2 Fiesta-2 Fiesta x Totem-2 PI 613988-2 Apple Integrated Map-2 M.27 x M.116-2
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02c06 gcatcaatgacgaaatccactactaatgcatttacagtagctagctgttcatctctctctctctctctctctctctctct ctctcatactagtcattctctgtttggttttgtgtatatgcttctctctctctctctctctctctctctctctctctctc tctcatactagtcattctctatttggttttgtgtatatacttctgcagggtgcatatataaaccttggcagataatatgt taaaaagcgcgcaatcattaacaa
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
236:240
236:240
230:246

230:254
250
250
216:250


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