CH02e02
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH02e02.F | CTCATCAGTCTCACTGACTGTGTG | 24 nt | 65.2 °C |  |
|
| CH02e02.R | AGGGTCAGGGTCAGTCAGG | 19 nt | 61.6 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | presumed single locus | ||||
| Detected loci | |||||
| Alleles size range | 122-130 | ||||
| Number of alleles detected | 2 | ||||
| Expected heterozygosity | 0.44 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 122 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Fiesta x Totem-13 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH02e02 aattggaagcaaaagagagagagagagagagagagagagagagcgcgcgctagtgaaagctangtaggaagagcaaggag tgtgtttgcaggcgagaaaactagaaacagcaaaaatagcaaatagtgttcagaaacctacacggcctctcacctcatca gtctcactgactgtgtgggggtggtgggaaagaggagagaggagagagagagagagagagagagcgagggagaagtgctt ttaagtagttaacaaccctgactgaccctgaccctaaaatt | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
122:130 122 122 122 122 122:130 122:130 |
||||
Send comments to Luca Gianfranceschi
