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CH03a02
Primer features
Name Sequence Length Tm  
CH03a02.F TTGTGGACGTTCTGTGTTGG 20 nt 58.4 °C Primer Stats
CH03a02.R CAAGTTCAACAGCTCAAGATGA 22 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 124-184
Number of alleles detected 9
Expected heterozygosity 0.87
PCR annealing temp 60
Sequenced allele size 148
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-14 Fiesta-14 Fiesta x Totem-14 Malling9-14 Robusta5-14 M.27 x M.116-14
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03a02 aattacagtgatggagaagatgggaggatgtttagggaacttgtggcggagttcatggagttactgggaagtgtctatat cggggactatatgccatcacttgcttggttgagccgtgtcaacggtatggatgcaagattggacaaggtggctaagaagt ttgatcattttttagagaaagttgttcaagaccatgtggtggatcatatctctaatgtggatagcaacaaggatcaaaag gattttgtggacgttctgtgttggattcggaaagaaaacttgattagttttcctattgaaacagttaccatatcatggta agcctctctctctctctctctctctctctctctctctctctctctctctctcatcttgagctgttgaacttgaaaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
138:160
124:184
128:160

126:158
124:150
144:160
144:150


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