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CH03b01
Primer features
Name Sequence Length Tm  
CH03b01.F ACAAGGTAACGTACAACTCTCTC 23 nt 61.1 °C Primer Stats
CH03b01.R GTCACAAAACCGCCAGATG 19 nt 57.3 °C
SSR info
SSR repeat type Imperfect
Locus type presumed multi-locus
Detected loci
Alleles size range 160-180
Number of alleles detected 6
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 176
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-2 M.27 x M.116-2 M.27 x M.116-15
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03b01 aattcgaacaaggtaacgtacaactctctctctctctctctcnctctctctctctctctctatttctctgtcattcgatc ttcgattaccgttgcaattgatgtgtaatggatctnggtangatccnaattgcaggcgtactttttcnganatggcgggc ggcgcatctggcggttttgtgacaaaancattcgantctaagctcaangagtgcgctccgaanancatgcatatcttcag aangctattcgcgcttatttacgtttcnttctgattcccttgtgaatacacttatgcataaacattgcgtnttcgcatgt attttttggactntggannatcangtgtttactttggttgngagattgttgaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
160:166:178
162:166:178
162:166:178

162:166:178
160:172:180
160:180
180


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