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CH03c02
Primer features
Name Sequence Length Tm  
CH03c02.F TCACTATTTACGGGATCAAGCA 22 nt 58.4 °C Primer Stats
CH03c02.R GTGCAGAGTCTTTGACAAGGC 21 nt 61.3 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 116-136
Number of alleles detected 5
Expected heterozygosity 0.65
PCR annealing temp 60
Sequenced allele size 134
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-12 Fiesta x A871-14-12 Discovery-12 Fiesta x Totem-12 Apple Integrated Map-12
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03c02 aatttatcaggggataacaagctacctagcgctgtctagtgttacacttttaatctatttgtatcatcatatgtatcatg tcactaacaaagatagagtttataaacatatgtagatctcatttatttctattataaaaataatacaaatagactataag aacaacatttttgctatattgtgccaaagaaaaagatgcgtctggggctgattcgaatgggcggcaacagaaatcttggg ggtgtgggggctacgatatgatgatcacatttatgcacagtaagggccctgatctcgtggtttgactttttgactcgttt ggggtcatttggtttgtccgttccgccgtggaanaaagatcgatgatatcactatttacgggatcaagcaaaagattgtc ctaccatattactctctctctctctctctctctctctctctctctctctctctctgggtatactttcaacgtgtgccagt tgccttgtcaaagactctgcacgtat
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
128
128:128
116:128

128:130
128:136
128:130
128:130


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