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CH03d01
Primer features
Name Sequence Length Tm  
CH03d01.F CGCACCACAAATCCAACTC 19 nt 57.3 °C Primer Stats
CH03d01.R AGAGTCAGAAGCACAGCCTC 20 nt 60.5 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 95-115
Number of alleles detected 7
Expected heterozygosity 0.79
PCR annealing temp 60
Sequenced allele size 112
Linkage group(s)
Developed by ETH Zuerich
Other maps A722-7 x Golden Delicious-2 Discovery-2 Discovery x TN10-8-2 Fiesta-2 Prima-2 Discovery-2 Fiesta-2 Fiesta x Totem-2 PI 613988-2 Apple Integrated Map-2
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03d01 aattcacccgttcacgtgagcctcacgtganttcgggtccCgcaccacaaatccaactcctcttctctgncatgctctcg aaggctctctctctctctctctctctctctctctctctctctctagctctgaGaggctgtgcttctgActCtcactctct ctctgtctctctcttcagctctgtgcgtgttggaaggtntgattggatgcnganagtgtgangtttcttggcgatgtgtg gaccaaactgtgtgngtctatctgaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
111:113
101:113
109:115

107
111:113
101:111
111


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