Select a marker

CH03d10
Primer features
Name Sequence Length Tm  
CH03d10.F CTCCCTTACCAAAAACACCAAA 22 nt 58.4 °C Primer Stats
CH03d10.R GTGATTAAGAGAGTGATCGGGG 22 nt 62.1 °C
SSR info
SSR repeat type Compound
Locus type single locus
Detected loci
Alleles size range 166-182
Number of alleles detected 6
Expected heterozygosity 0.76
PCR annealing temp 60
Sequenced allele size 172
Linkage group(s)
Developed by ETH Zuerich
Other maps Regia x Pingo-2 Regia x Piflora-2 Discovery-2 Fiesta-2 Fiesta x Totem-2 Royal Gala-2 Malling9-2 Robusta5-2 Apple Integrated Map-2 M.27 x M.116-2
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03d10 aattaccctctctctctctctctctctctctctctctctctctctctcccctcccccttccccttctctcccttaccaaa aacaccaaaacaactctctctctctctctctctctcccccctttcctttctctctctgtttaagttcttctctcatggtt tctcaataaaacccttatctttctccacactctcactctccgggtactgcaatcagaccccgatcactctcttaatcact cgctttacggtacgattttcggttaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
166:168
166:182
166:nul

170:172
172:182
172:182
172


admin Send comments to Luca Gianfranceschi