Select a marker

CH03e03
Primer features
Name Sequence Length Tm  
CH03e03.F GCACATTCTGCCTTATCTTGG 21 nt 59.4 °C Primer Stats
CH03e03.R AAAACCCACAAATAGCGCC 19 nt 55.2 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 106-216
Number of alleles detected 6
Expected heterozygosity 0.78
PCR annealing temp 60
Sequenced allele size 198
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-3 Fiesta-3 Discovery-3 Fiesta-3 Fiesta x Totem-3 Royal Gala-3 Malling9-3 Robusta5-3 Apple Integrated Map-3 M.27 x M.116-3
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH03e03 aattaggacacacaggcatttcttgctcttctgcaccactactttaatcccactgcnacantctatggttggacttttgg catcgccgccgacgtacggaaggcagggggcaagccctactagttggtctgcacattctgccttatcttggtctatgttg cagctgccaanaccaactataaatatcaacaccaaggtgcatganagtacctgcacactcacattctttgagttattcat tgtngtgcanatagagagagagagagagagagagagagagagagagggagggtggganagaanaaatggggcgctatttg tgggttttgtgctttttcttggctgcccttttggtttntatncaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
186
198:206
198:216

186
206
186:206
186:204


admin Send comments to Luca Gianfranceschi