CH03e03
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH03e03.F | GCACATTCTGCCTTATCTTGG | 21 nt | 59.4 °C | ||
CH03e03.R | AAAACCCACAAATAGCGCC | 19 nt | 55.2 °C | ||
SSR info | |||||
SSR repeat type | Imperfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 106-216 | ||||
Number of alleles detected | 6 | ||||
Expected heterozygosity | 0.78 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 198 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Discovery-3 Fiesta-3 Discovery-3 Fiesta-3 Fiesta x Totem-3 Royal Gala-3 Malling9-3 Robusta5-3 Apple Integrated Map-3 M.27 x M.116-3 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH03e03 aattaggacacacaggcatttcttgctcttctgcaccactactttaatcccactgcnacantctatggttggacttttgg catcgccgccgacgtacggaaggcagggggcaagccctactagttggtctgcacattctgccttatcttggtctatgttg cagctgccaanaccaactataaatatcaacaccaaggtgcatganagtacctgcacactcacattctttgagttattcat tgtngtgcanatagagagagagagagagagagagagagagagagagggagggtggganagaanaaatggggcgctatttg tgggttttgtgctttttcttggctgcccttttggtttntatncaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
186 198:206 198:216 186 206 186:206 186:204 |
Send comments to Luca Gianfranceschi