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CH04c03
Primer features
Name Sequence Length Tm  
CH04c03.F TGCACACCAAACACAGGACT 20 nt 58.4 °C Primer Stats
CH04c03.R TATCAAACATTGGGGCACTG 20 nt 56.4 °C
SSR info
SSR repeat type Compound
Locus type presumed single locus
Detected loci
Alleles size range 194-198
Number of alleles detected 3
Expected heterozygosity 0.41
PCR annealing temp 60
Sequenced allele size 199
Linkage group(s)
Developed by ETH Zuerich
Other maps Robusta5-5
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04c03 aattggggttcttcgttaagaactcgtaaggaggcgtgtaatccgagcgagtcccacctaagcccattaaagctaatccg gttgcacaccaaacacaggactatagtctagtccagtccaagccttgctaatcctgtcaaacaaacgtgcccttaggggn tgggnggccatgcctccaatagagagagagagagagagacagatagcgagcgatagagagagagagagagagggaggggg agagggaganattaggatgttcagtgccccaatgtttgatatggtgactgggttgggttgacagtgaaanaaagaggatg gttcctcccgattacggggaaanaacaaaaagggaagggtggggattggttaaacaggcccancttgntccccccttccg gaatcctccccantcttccnncccgttgcctntttccaaaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
198
198
198

196
198
198
198


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