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CH04e05
Primer features
Name Sequence Length Tm  
CH04e05.F AGGCTAACAGAAATGTGGTTTG 22 nt 58.4 °C Primer Stats
CH04e05.R ATGGCTCCTATTGCCATCAT 20 nt 56.4 °C
SSR info
SSR repeat type Perfect
Locus type presumed multi-locus
Detected loci
Alleles size range 174-227
Number of alleles detected 8
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 200
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-7 Fiesta x Discovery-7 Prima x Fiesta-7 Braeburn-4 Telamon-4 Discovery-7 Fiesta-7 Fiesta x Totem-7 PI 613988-7 Malling9-7 Apple Integrated Map-7 M.27 x M.116-7
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04e05 aattcagttgctgcctcggagggagagagagagagagagagagagtttaaacttggaaaagagaaggctaacagaaatgt ggtttgtgagaaataaagttcttgttctttaaataggagagatgtctgggcagaacccaggagacaaagggtgagccaca tggctatttttgtgtttgtatctgtncttganagagagaganagagagagagagagagagaganagagagggctttccnt antnatgatggcaataggagccatgattacaaaggccnattaaaatgattgaaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
174:181:209
199:227
197:201

174:181:201
181:201
181:203
174:181:203


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