CH04e12
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH04e12.F | CCTGAAATCTGCACAACTACCA | 22 nt | 60.3 °C | ||
CH04e12.R | GGTGGTGAAGAAGTAGACAGCC | 22 nt | 64.0 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | presumed single locus | ||||
Detected loci | |||||
Alleles size range | 226-234 | ||||
Number of alleles detected | 3 | ||||
Expected heterozygosity | 0.34 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 224 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Fiesta x Totem-2 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH04e12b aattatcacccaccacttttcttactgtgcatctctctctctctctctctctctctctctctctctctttgtccatacgt gaaatctaaaaggagtagaaggtcactgtaatggcctgaaatctgcacaactaccattcttttttgtttcttccgctcac acccaccaccgccatcaccaccaccatgtagactcttttcctctctcttactcttctctctctctctctctctgaacaca aacaagtacattcttttcctacaacaaagcacagcacaatgccagccctcaatctgctgatgatcctcttgctcacggct gtctacttcttcaccaccgccacttgttaccaccacaaccgccacctcctccaccagccctttttccccttatccnnncc ccctccaacccaacccccatcctcctctccccaatcccagccctcctcaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
226:230 226 226 226 226 226 226 |
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