Select a marker

CH04e12
Primer features
Name Sequence Length Tm  
CH04e12.F CCTGAAATCTGCACAACTACCA 22 nt 60.3 °C Primer Stats
CH04e12.R GGTGGTGAAGAAGTAGACAGCC 22 nt 64.0 °C
SSR info
SSR repeat type Perfect
Locus type presumed single locus
Detected loci
Alleles size range 226-234
Number of alleles detected 3
Expected heterozygosity 0.34
PCR annealing temp 60
Sequenced allele size 224
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-2
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04e12b aattatcacccaccacttttcttactgtgcatctctctctctctctctctctctctctctctctctctttgtccatacgt gaaatctaaaaggagtagaaggtcactgtaatggcctgaaatctgcacaactaccattcttttttgtttcttccgctcac acccaccaccgccatcaccaccaccatgtagactcttttcctctctcttactcttctctctctctctctctctgaacaca aacaagtacattcttttcctacaacaaagcacagcacaatgccagccctcaatctgctgatgatcctcttgctcacggct gtctacttcttcaccaccgccacttgttaccaccacaaccgccacctcctccaccagccctttttccccttatccnnncc ccctccaacccaacccccatcctcctctccccaatcccagccctcctcaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
226:230
226
226

226
226
226
226


admin Send comments to Luca Gianfranceschi