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CH04f10
Primer features
Name Sequence Length Tm  
CH04f10.F GTAATGGAAATACAGTTTCACAA 23 nt 55.7 °C Primer Stats
CH04f10.R TTAAATGCTTGGTGTGTTTTGC 22 nt 56.5 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 144-254
Number of alleles detected 9
Expected heterozygosity 0.88
PCR annealing temp 60
Sequenced allele size 200
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-16 Discovery-16 Fiesta-16 Fiesta x Totem-16 Malling9-16 Robusta5-16 Apple Integrated Map-16
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH04f10 aattagttaactttggtgtttatgtataaatactgattgtacacataatcttaatgtaatggaaatacagtttcacaatt ctctctctctctctctctctctctctctctctctctctctctctctctctctctctgatgccttgccaatgcttagcaaa gagagcaacgctttcccttcattcatttgttgggtttgataaaaggatggcatgtctagagatacggcagaccgcaaaac acaccaagcatttaaaagactcatacatgtgtgtatgcagtaaagagattctctcattctcaggtcttaanaanttagtg ccaatgaaggtattttctgtttatctatcttttacttctcaaactgaaaaactcaatgcatgcataactaacctattata tagtgtaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
175:238
232:244
244:246

238
195
254
195:144


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