CH04h02
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH04h02.F | GGAAGCTGCATGATGAGACC | 20 nt | 60.5 °C |  |
|
| CH04h02.R | CTCAAGGATTTCATGCCCAC | 20 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 162-262 | ||||
| Number of alleles detected | 14 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 179 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery-11 Discovery x TN10-8-11 Discovery-11 Fiesta-11 Fiesta x Totem-11 PI 613988-11 Apple Integrated Map-11 M.27 x M.116-11 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH04h02 aatttctggttgttggaaaaaggncttccttgttagtgtaagnggaagctgcatgatgagaccttctttcgtccatagtg taaaacaaggccttgaaaactcatgatttctaaagaaaatgaaaatgagaatataaaagngagagagagagagagagaga gagagagagagagagagagagatggaagagagagaagagaatgtgggcatgaaatccttgagtaaatcaggtagtagtcc tcctccttcaggttcaaatccacggtggatgcaacattactggccgtcggtgaagccatttgtgttacggtggagctgct gggttttttacaagtttcctcgcttttgccagttcggccaccctcttctattccccatctccagacattgtcgctcgctt tcnaaacactcccccattccntttgttttcccaaaaaaaacatcaggttcccattggaatccctgcncccgggggattcc nctatttcttaaagcggcccccccgcgggtggaaccccccctc | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
174:186:190:224 166:176:224:240 174:182:194:208 174:176:190:224 174:176:194:224 174:182:190:206 172:174:194:262 |
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