Select a marker

CH05a05
Primer features
Name Sequence Length Tm  
CH05a05.F TGTATCAGTGGTTTGCATGAAC 22 nt 58.4 °C Primer Stats
CH05a05.R GCAACTCCCAACTCTTCTTTCT 22 nt 60.3 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 198-230
Number of alleles detected 6
Expected heterozygosity 0.77
PCR annealing temp 60
Sequenced allele size 219
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-6 Fiesta-6 Fiesta x Totem-6 PI 613988-6 Apple Integrated Map-6 M.27 x M.116-6
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05a05 aattgacggagggaggatgaaaccctagctatgtatcagtggtttgcatgaaccggagcgagagagagagagagagagag agtgggtggtattggtggagtggggctctcgatatctgtgtgctgggaatatggatggatatatatggactctgtgtgtg tgatgagtgatgagagagagagagagagagagagagagagagagagagagaganatcttttgcttttgagaaagaagagt tgggagttgcggcttgatggtttgcttgcttgtccgagccaagaaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
198:218
216:230
214:230

216:218
216:218
216:218
214:218


admin Send comments to Luca Gianfranceschi