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CH05b06
Primer features
Name Sequence Length Tm  
CH05b06.F ACAAGCAAACCTAATACCACCG 22 nt 60.3 °C Primer Stats
CH05b06.R GAGACTGGAAGAGTTGCAGAGG 22 nt 64.0 °C
SSR info
SSR repeat type Imperfect
Locus type multi-locus
Detected loci
Alleles size range 138-226
Number of alleles detected 14
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 170
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-10 Discovery x TN10-8-5 Discovery-5 Fiesta-5 Fiesta-16 Fiesta x Totem-10 Fiesta x Totem-16 Apple Integrated Map-5 Apple Integrated Map-10 M.27 x M.116-3 M.27 x M.116-10
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05b06 aattccttatgcacggtacatccatctcccccagttgtctccccccctctctcccctcatcagaacccagaaaaaacaag caaacctaataccaccgccaaacacacacccaacacaccctgggacctccttctgcatctctctctccctctctctctat ctctctctctctctctctctctctctctctctctacccatttcgctctgcacccctggaactccctctgcaactcttcca gtctccccacagaaaaatgaaaatccgcaccttgggttttttccttggtttgccgttgctttctgggttttgttttatat gtttgtttttgtttatgctgttagctccggaaatccaatgtttgtcacccgggaaaaaaactccaacgaaaatt
User's notes
Luca Gianfranceschi (luca.gianfranceschi@unimi.it)
Date 24 November 2005 - Time: 17:23
This markers has been mapped only on the alternative maps Calenge_TAG110_DT5==>CH05b06a and Calenge_TAG110_DT10==>Ch05b06b.

Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
172:174
164:174:220
138:160:174:226

154:164:174:220
164:174
140:164:174:214
164:222


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