Select a marker

CH05c07
Primer features
Name Sequence Length Tm  
CH05c07.F TGATGCATTAGGGCTTGTACTT 22 nt 58.4 °C Primer Stats
CH05c07.R GGGATGCATTGCTAAATAGGAT 22 nt 58.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 111-149
Number of alleles detected 7
Expected heterozygosity 0.84
PCR annealing temp 60
Sequenced allele size 152
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-9 Discovery-9 Fiesta-9 Prima-9 Discovery-9 Fiesta-9 Fiesta x Totem-9 Robusta5-9 Apple Integrated Map-9 M.27 x M.116-9
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05c07 aatttattcaatccgatgatgaaacttaaagagaagcgtggaagaagaaaaaaaagtcttagattatccagcctatcaac tagtctgatgtttgaaacaatcttcatagtttgatattttaaagtcaatctttattattcatttttcgagctaagaaaag ttgaaaaagtgtttagttatcatttgctttttacaatgcatgatgcattagggcttgtacttcatattgtgattggttct ctctctctctctctctctctctctctctctctctctctccctctctccctcccctccagttggaaaaagaaaaagaaaag ccagaagttgatcctatttagcaatgcatccctattccagctttctgcccaacttttgcttttgccccacaacttgcacc ctaaaatgacatgggggatgtttgtgccataaatctcccgtggaaaataagcttgttcattttgttgatacaccacttta aacttttaatccgtgaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
141:149
125:141
113:139

125:135
141:149
111:139
113:149


admin Send comments to Luca Gianfranceschi