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CH05d08
Primer features
Name Sequence Length Tm  
CH05d08.F TCATGGATGGGAAAAAGAGG 20 nt 56.4 °C Primer Stats
CH05d08.R TGATTGCCACATGTCAGTGTT 21 nt 57.4 °C
SSR info
SSR repeat type Compound
Locus type multi-locus
Detected loci
Alleles size range 91-143
Number of alleles detected 10
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 145
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta-17 Discovery x TN10-8-17 Discovery-9 Discovery-17 Fiesta-17 Fiesta x Totem-17 TN10-8-17 Royal Gala-17 Apple Integrated Map-17 M.27 x M.116-17
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05d08 aattagtttcatggatgggaaaaagaggtggagagagagagagagagagagagagagagagagagagagagagagagaga gcatatatcaggagaggttatcactttagtcagctaactgtaacctaatcacaacactgacatgtggcaatcagtataat aaaaactctcaaaataaaagcttcaaaaaagttatcctcaagcttagtgagttatgaaagaatcagcattgtaaaatcca tgatatacacttcttcaacactggtattccagagttaagaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
110:122
106:110:122:124
91:110:120:122

106:122:125:135
110:122:143
110:122:137
122:143


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