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CH05e04
Primer features
Name Sequence Length Tm  
CH05e04.F AAGGAGAAGACCGTGTGAAATC 22 nt 60.3 °C Primer Stats
CH05e04.R CATGGATAAGGCATAGTCAGGA 22 nt 60.3 °C
SSR info
SSR repeat type Perfect
Locus type presumed multi-locus
Detected loci
Alleles size range 153-234
Number of alleles detected 9
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 164
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery x TN10-8-16 Discovery-16 Fiesta-16 Fiesta x Totem-16 Apple Integrated Map-16
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05e04 aatttcccaaggnaaacaatccgtctgacttgatatttagatgactgtaacatggacaagtggatatgacatcgtggaag caaccccttttgttgagtggggtataaaaggagaagaccgtgtgaaatccccagctgggacattgaacttcgaccgcaac accatgtgtggtgtgtttgctctctctctctctctctctctctctctctctctctctctctcttgggggggatggttgag ggttgcatctcctgactatgccttatccatgttacgttctacaaacacaggttgcacccggcaaaaccgtggggttggcg aagatcctggatttatacacactgctttctgaaagaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
159:230:234
153:167:230
153:161:230

159:175
167
157:167
167


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