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CH05f06
Primer features
Name Sequence Length Tm  
CH05f06.F TTAGATCCGGTCACTCTCCACT 22 nt 62.1 °C Primer Stats
CH05f06.R TGGAGGAAGACGAAGAAGAAAG 22 nt 60.3 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 166-184
Number of alleles detected 5
Expected heterozygosity 0.74
PCR annealing temp 60
Sequenced allele size 175
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-5 Discovery-5 Fiesta-5 Fiesta x Totem-5 Malling9-5 Robusta5-5 Apple Integrated Map-5 M.27 x M.116-5
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05f06 aattactagttactacctacaaatacaagttactagttattcgttgcttcgttggaactagtgttgacattagatccggt cactctccactgaagtaaagaaactctctctctctctctctctctctctctctctctccacccgcgtgtgacaganaagt gctttgtcatccacgacccctgccttttactttgactccaactgcgtgcttctcaggtacttctttcttcttcgtcttcc tccattatctctctccttctttacaccttttccattttttaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
180:184
166:176
166:184

180:184
176:180
176:184
176:184


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