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CH05g11
Primer features
Name Sequence Length Tm  
CH05g11.F GCAAACCAACCTCTGGTGAT 20 nt 58.4 °C Primer Stats
CH05g11.R AAACTGTTCCAACGACGCTA 20 nt 56.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 201-255
Number of alleles detected 6
Expected heterozygosity 0.78
PCR annealing temp 60
Sequenced allele size 250
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-14 Fiesta-14 Fiesta x Totem-14 Apple Integrated Map-14 M.27 x M.116-14
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05g11 aattaaacgagcaaggtaagagcaaaccaacctctggtgatcggaataaggaatggttctgaggagggagatttcttgaa gtctcttttcttttctttctttggctctttcagatttttccatgtctgctctgcgctctgcgtcttgagagagagagaga gagagagagagagagagagagagagatagagagagagatggagagagatagagagagatagagagtgagagagcgtatgt tttggttagtttagcgtcgttggaacagttttatcacagccgccgcggctcctaggagcgacaggactccgtcatttgcc gcgttgttggttttggtcaattgcctgcccgttgttccttcctccaaaactccccgcccataatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
201:243
201:243
213:255

201:213
243:247
201:247
229:243


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