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CH05h05
Primer features
Name Sequence Length Tm  
CH05h05.F ACATGTCACTCCTACGCGG 19 nt 59.5 °C Primer Stats
CH05h05.R GTGCAGTGATTAGCATTGCTGT 22 nt 60.3 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 168-184
Number of alleles detected 4
Expected heterozygosity 0.75
PCR annealing temp 60
Sequenced allele size 180
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-13 Fiesta x Totem-13 Royal Gala-13 Malling9-13 Apple Integrated Map-13
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH05h05 aatttgccacatgtcactcctacgcggtctctctctctctctctctctctctctctctctctctcacattggtagcatct atatatatgtaccttgcacgtacctcgttggcaaacaaaaacatacatcaaactttggaaaagctaaaagcttagtaacc atgtctacagcaatgctaatcactgcacttcactcagctcttccaccgccgcggcatctcaactccggctgtaaccgaaa gacggggttgttggaaatacggcgttangcattgtcctatgacgatttctgcgttcgtcgcgttggtccatgataactac gaaggcagacttgttggacgaaagacatgattgttgcttccaaatgcgaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
168:184
174:174
168:184

174:180
180:184
168:180
180


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