CN493139-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CN493139-SSR.F | CACGACCTCCAAACCTATGC | 20 nt | 60.5 °C |  |
|
| CN493139-SSR.R | GTTTATGAAAGTACGGCACCCATC | 24 nt | 63.5 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | presumed multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 124-162 | ||||
| Number of alleles detected | 10 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-2 Fiesta-2 Fiesta x Totem-2 Fiesta x Totem-5 Royal Gala-2 Malling9-2 Apple Integrated Map-2 M.27 x M.116-2 M.27 x M.116-5 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean | ||||
| Sequence | |||||
| >gi|46612706|gb|CN493139.1|CN493139 Mdfw2012f06.y1 Mdfw Malus x domestica cDNA clone Mdfw2012f06 5' similar to TR:O81808 O81808 HYPOTHETICAL 62.6 KD PROTEIN. ;, mRNA sequence GTACACGACCTCCAAACCTATGCATTGTGACAGGTTTATCTTAGAGAATATATATATATATATATATATA TATATATATTAATGATTCTCATGTGCACAAGTATCTTATTTCTTGAGTAGTCATGATTTCTATGGATGGG TGCCGTACTTTCATCTTTGTTATACCATGTCTAATTGGTTACAACTGAAGAGAATAGTTATATATTGATT GTCAACACTTACGAATTTTAATTTAATGCGATTTGAATTCACTGATGACCACCTTTTGATGCTCATGTTC CTGCTTATCAGAATTTATGTCCAAAGGAAGTGTATACGATTTTCTGCATAAGCACAAGGGTGTGTTTAAG CTTCCATCTCTACTCAAAGTAGCAATTGATGTCTCCAAGGGAATGAACTATTTGCACCAAAATAATATAA TCCACAGGGATCTTAAGACTGCCAATCTTCTTATGGATGAACATGAAGTCGTTAAGGTTGCTGATTTTGG GGTTGCCAGAGTGCAGACTCAATCTGGAGTGATGACAGCTGAAACTGGTACATACCGCTGGATGGCTCCT GAGGTC | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 14:11 Sequence Assembly Library View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
124:144:156 124:136:142:150 140:144:162 126:140:142:162 124:126:140:162 124:142:146 124:126:142:144 124:140:156:162 |
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