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CN496002-SSR
Primer features
Name Sequence Length Tm  
CN496002-SSR.F TCAGAATCTCAAGCAAGATCCTC 23 nt 61.1 °C Primer Stats
CN496002-SSR.R GTTTGATTGATCGTGGCGATATG 23 nt 61.1 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 243-261
Number of alleles detected 4
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-5 Fiesta-5
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks clean
Sequence
>gi|46597951|gb|CN496002.1|CN496002 Mdfw2021d09.y1 Mdfw Malus x domestica cDNA clone Mdfw2021d09 5' similar to TR:O23131 O23131 HYPOTHETICAL 37.1 KD PROTEIN. ;, mRNA sequence AAAATCACCATTCAGAATCTCAAGCAAGATCCTCTTCTTCTTCTTGTACCGCCGCCGCCACCACTAACAG CACCAGCAGCAGCTAGGCTAGAGCTAGATCTATTGTTAGTGTAGTGCATACCAATTATTAGTAGCTAGAT ATATATATATATATATATATATAGGACTCAATTCGTAATTAGTAACATCAACACCACCGCACAGGATTTC TAAGAGATCGATCGAGAGCTGATCATATAATCATATCGCCACGATCAATCAAGATTTGAAGATGACTCCA GCTAATCTGGCAAGGCAATTTGGCGACACAACTTACACCAAAGAGTTTGTTGGAGGGTTGGCATGAGAAA CCCAGAAGGAAACCATGAAGAATAACTTTGAA
User's notes
LucaG (luca.gianfranceschi@unimi.it)
Date 13 February 2006 - Time: 14:14

Sequence
Title: Mdfw2021d09.y1 Mdfw Malus x domestica cDNA clone Mdfw2021d09 5' similar to TR:O23131 O23131 HYPOTHETICAL 37.1 KD PROTEIN. ;, mRNA sequence.
Type: mRNA
Length: 382 bp
Organism: Malus x domestica
GenBank Accession: CN496002

Assembly
This sequence was assembled into contig

Library
Name: Mdfw
Cultivar: GoldRush
Tissue:Flower

View Sequence Info at PlantGDB



Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
243:257
243:259
243:259
257:261
nd
243:261

243:257

243:257


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