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CN581493-SSR
Primer features
Name Sequence Length Tm  
CN581493-SSR.F GCTTTTCATGGTGGAAAAACTG 22 nt 58.4 °C Primer Stats
CN581493-SSR.R GTTTGACTCTCCGCTCTGATGGAC 24 nt 66.9 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 184-228
Number of alleles detected 8
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-2 Malling9-2 Robusta5-2 Apple Integrated Map-2
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks clean
Sequence
>gi|46993043|gb|CN581493.1|CN581493 Mdfw2039o14.y1 Mdfw Malus x domestica cDNA clone Mdfw2039o14 5' similar to TR:Q9ZQF5 Q9ZQF5 PUTATIVE RING-H2 FINGER PROTEIN. ;, mRNA sequence CAGCTTTTCATGGTGGAAAAACTGTATCATTTATAAGAGAAATATATACATATATATATATATATATATA GCTACACTTGTTTTCTTTGTCGGGTTTTGAGTTGTTGTAATTTGGAGGTTGAAATTAATGGCTGGAATGC TTCCTGGTGTGGAATGCGCTCGGAGGAGACGAGTCCATCAGAGCGGAGAGTCACCGTGTGTCGGCGGCAT CCATGGCAGCACCAGGCGCTCTTCTTTCTGTTTGTATGCTAGCAACCATGAATCTCACCACACCTCCACC TCTTCACTGCAAAGAAGCATATTGAACCAAGCATACATGGATGAGAAGCTGGGAGTGGAAGCCACAAAAG CCAAGGGGAAAATACACGAAAGAAAGAGAACACAAAGAAAACCACAATAC
User's notes
LucaG (luca.gianfranceschi@unimi.it)
Date 13 February 2006 - Time: 14:18

Sequence
Title: Mdfw2039o14.y1 Mdfw Malus x domestica cDNA clone Mdfw2039o14 5' similar to TR:Q9ZQF5 Q9ZQF5 PUTATIVE RING-H2 FINGER PROTEIN. ;, mRNA sequence.
Type: mRNA
Length: 400 bp
Organism: Malus x domestica
GenBank Accession: CN581493

Assembly
This sequence was assembled into contig

Library
Name: Mdfw
Cultivar: GoldRush
Tissue:Flower

View Sequence Info at PlantGDB


Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
 		194
184:190:194
194:218
228
194:218
192:220

194:226

194:218

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