EMPc104
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| EMPc104.F | ATCTTTCACAAGCATATCGACG | 22 nt | 58.4 °C |  |
|
| EMPc104.R | GAGGACACTTCTAGCAACAGAAAA | 24 nt | 61.8 °C | ||
| SSR info | |||||
| SSR repeat type | unknown | ||||
| Locus type | |||||
| Detected loci | |||||
| Alleles size range | 87-123 | ||||
| Number of alleles detected | 9 | ||||
| Expected heterozygosity | 0.84 | ||||
| PCR annealing temp | 0 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | East Malling Research (UK) | ||||
| Other maps | Fiesta x Totem-15 M.27 x M.116-15 | ||||
| Reference publication | Fernandez-Fernandez, F; Harvey, NG; James, CM (2006) Isolation and characterization of polymorphic microsatellite markers from European pear (Pyrus communis). Molecular Ecology Notes 6(): 1039?1041 | ||||
| Remarks | polymorphic amplification in Malus | ||||
| Sequence | |||||
| >gi|118402599|emb|AM182389.1| Pyrus communis satellite DNA, clone EMPc104 ACTGGGTTTGAAGGCTTATCTCCATATTTCTTCACAAATTTCTTTGGAATACTCTGTGCACAACAGAGAT CGTGTTAACTAACTGGTGATAAATTTAAAAGGATGAATTATCGGCTATATTTGTAGCTAAAAAATGAATC ATCTCAAACAATATTGTGGCTAACAATATTATTTAATAGCATAGTGAAGGCAAGAAACATCTTTCACAAG CATATCGACGGAGACCCGTTTCTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG GTGCTTACTAGTTTTCTGTTGCTAGAAGTGTCCTCTAGGATGGCTTTAAAAAAATGGGGAGGCCAGTTAT CAGTTTTCCCGTG | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Not available |
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