Select a marker

Hi03a03
Primer features
Name Sequence Length Tm  
Hi03a03.F ACACTTCCGGATTTCTGCTC 20 nt 58.4 °C Primer Stats
Hi03a03.R GTTTGTTGCTGTTGGATTATGCC 23 nt 61.1 °C
SSR info
SSR repeat type Perfect
Locus type presumed multi-locus
Detected loci
Alleles size range 160-228
Number of alleles detected 10
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-6 Fiesta-6 PI 613988-14 Robusta5-14
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks clean
Sequence
>Hi03A03 CGAGCGTTGGTGGCGAAGAAGTTTCTAAAGGTGACGTTGCTGTTTGCGGCAGCCACTGTG TGTTGCTTTGGCTTCTACAATTCTGTCTACACTTCCGGATTTCTGCTCAGATCCTCTTAT GACTACGCNTTTGTCTGGCACCAGAACCGTAACTTCTCATCAGGCCAGGACTCTCTCTCT CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGCATGTTCTATTTTGTGTTCATCCC AAGGCTTGTTATTTTAGTCATAATTAAGCCTTGTTATTTTAGTCATAATTAAGCCGGCAT AATCCAACAGCAACATGTTTTTTTTTATTAGTTTTTCTAAAAAACGATCAATTACCCATA AAATATCAAAGTGGTAGTAATTACTTTTAGACTCTTGAGCAAATTAAGTAAAACAATATA TTCTTGTTGTATAGCTAATCCGAAATCAATATATACATAATTATGAAGGATTCGGCAGTC AGATAATTATGTTTATCGCGAGGAGCCAGATCTGAAACAATCACTAGTGAATTCGCGGCC GCCTGCAGGTCGACCATAT
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
188:192:224
172:196:224:228
160:172:224:228
160:228
160:224:228
160:172:228

186:194:218:228

nd


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