AF057134-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| AF057134-SSR.F | ACTACCCAATGCCCACAAAG | 20 nt | 58.4 °C |  |
|
| AF057134-SSR.R | TATCCTCGCCCAAAAGACTG | 20 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 202-224 | ||||
| Number of alleles detected | 7 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-10 Fiesta-10 Apple Integrated Map-10 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean, compl. band(s) | ||||
| Sequence | |||||
| >gi|3688804|gb|AF057134.1|AF057134 Malus domestica NADP-dependent sorbitol 6-phosphate dehydrogenase (S6PDH) gene, complete cds ATGTCCACCGTCACCCTGAGCAGTGGCTACGAGATGCCGGTCATCGGTCTCGGCCTTTGGCGTCTGGAGA AGGACGAGCTTAAAGAGGTCATCTTAAATGCTATTAAGATTGGCTATCGCCATTTTGACTGTGCTGGTAT ACCCTCCATCCCTCACCTCCTCGTTTTTTACATTATTCATGCATGTCTACATATTATTCTGCTTTATATT CAACCGCAAAGAAAGGGGATGTGTTTGTTAATCCTTGTTTTATTTTTCACTTACTTCATTCCGTATTTAT CTTTTTTTAACACAATCGATATTAAAGAAGGGATAATCAAATATTAGACTTTGACTATTGAACAATAATG CTCCCTTGCTAGTATTAATCAATATTCTTACTCATAGCTGTCATATTATAGAACCCATCGTTTTATGTCT ACGTACACGGCTACGGATTGAGGTCATTATCAGAAAAAACGACCCAACCTTTCAAGGGATTTCACTTTAC CTAGCTGAAGTACTAAAGGGTTTAACTGGTTTTTTTCCATTAGCAATTTGGTAAAGGAAATCACATGCAT CATGCACAGAAGATTTTTCAGAAACAAGAAAAGAGTGTACACGCCATAAAGCAGCGGTGCTGCAATGTTA ACGTAACTATTTTTGGTCACATGTAACTATCAAATAAAAAAATGGATGTAGTTTGTAAAGGAGTCAAAAC CATGGGAACCCCGTTTGTTACTTTTATAGTTTAGTAAAATTAATTAATATATGTGTATTTATATATTCTG AGAAAAATATTACTATATGTCGCTAGCCACTGTATGCTTATGTTACTGCATCCATGCATTTGAGATTTTG ATTTTCTTTTTCTCAATATTATTTGGTTGAAAAATTATGATAATTCGTTTGAGGTCATAAAATATTTTCA ATACCTTATATCAATAAACATCTTGTGCTTTTAAACGAAGTCGATGAAGAGAAAGTTTTTAAGTGGTATT CTTTAATTGAGGAGGAAAAATTGTTTTCATTTAGCTTAGTTAAAAAGAAAATTATTTATATTGACCATGT TAGAAATGAACTTGACTTATGCAAACATATGAATTTTTATAAACAAAACAATCAAACAAAAACACAGGTT TGGTTACTATGAGTTGTATAAATAAGTGTTTAATCTTTTACTTTTTTTAGTTTAGGCCGAAAAGGGTATT ATGGTTTTTTCCCAAAAATGGCAGTTTGTCTTTTAGGACGCGATTAAGTGCACCTTCACTAAAGAAAATT TTTAGTGTGTCGAGAACACAATTAGTACGCTATCTGTAATAATACAATTAGTTAAAAATTTAGAAAAAAA TTGCTACCAATTAAATTAGGAAATTGTTATTATCACTAAAAAAATTTCATTCTACACTCATCATATATGT ATTTTTTTCTAATTATAAAAAATTTAAAGTACCAAAAAAAATTATTAAAGTGCTAATAATAATTTTCTTA AATTATTTCACTTATTGTATTCGACTGTGTTCTCGAAACTCTGAAAATTTATACATCCGAGAGCACAACA TGACGAACTAGCTAAGGTACTGTTGTGTTCTGGTGGACAAGACAAATGAGTGGCATACCTTGGTTCCCTA TGTTTTTGGACCAATCCCGAGTTTGCGTCTCACCTTGGGGAAAAGGAATAACCATAATCCAGTCCCTTGT TTGCTTCTTACCTTGGGATGAGGAATAATCCAAGTTCCCCCCCCATGGTTGCTTAATTTGCTTCTCACCT TGGGAAATAAAATAATAATAATACTAATAATAAACGCGAGAGAGTGGTCAGAAGAAGAGTGTCTTAATTA TCAGGAACAATTAACTACTTGCAAAGTTGATTTTTAGAATAAAAACCAGACATTTTGGTCTTCATGTTGT AAATTTAATTTCAGATTAGTTATTGTTGTATGCTTAATAATGGTAAATTATTTTGATATCGCAGCTCATT ACAAAAGTGAAGCAGACGTTGGAGAAGCACTTGCAGAAGCATTTAAGACTGGACTTGTTAAGAGGGAAGA ACTTTTCATTACCACCAAGGTCGGTTTAATTTATTTACCCTTTTTTATTTTTTTATTTTGCTACTTTTAT AAAATAAAATTGGTGTGACAAATTTAGGCTACGCCACAAAACGGACCGGTGATGACCAAGTATTAAGTCG TCATTTATAAGAGTCAAATCTAATACTCTTACTCATGAATGAACAAAAATATATCAAGATTGCAACGCAA ACAACGAAGTATTAGAGTTTATCTCACATTTTCCAAAGAGACTAAAATATAATTTTGTTGTTCTTATATT GCAGATTTGGAATTCAGACCATGGGCATGTGGTGGAGGCCTGTAAGAACAGCCTCGAGAAGCTTCAGATA GATTATCTGGATCTCTACCTGGTTCACTACCCAATGCCCACAAAGCACAATGGTACCTCTCTCTCTCTCT CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGGTTAGATTATTGTGTCACTCAACGCTGTAACGTTACA TAATTATAAATAGTTAATTTACAATGAATTGGTATTTATTTTGCTTTGCTGTATGGTCACAGCAATTGGT AAAACTGCCAGTCTTTTGGGCGAGGATAAGGTGTTGGACATCGATGTAACAATTTCCCTTCAACAAACCT GGGAGGGCATGGAAAAGACCGTCTCTTTGGGCTTAGTTCGCAGCATTGGTCTCAGGTACATCCCATAAGC ATTTATGCTAGACTTTCATCTATGTATCCAAACTCGATTATCTAGAATTGACCAGTGAATGTCACTAAAC CAAATGATCATCGGCTTCCGTTTTGCAGCAACTATGAGCTCTTTCTAACTAGAGATTGCTTGGCTTACTC CAAAATAAAGCCTGCTGTGAGCCAATTTGAAACCCACCCCTATTTCCAGCGCGACTCTCTCGTCAAATTC TGTATGAAACACGGCGTTCTTCCCACAGCTCACACCCCTCTCGGAGGTGCTGCTGCCAACAAGGATATGT TTGGTTCTGTTTCACCTTTGGATGATCCAGTTCTCAATGTAAGTCTTCTCTCTTTTAGCACACACACCAT ATGGAATACAACTGGATCGTAGCGCTAGTAAAAATACAACCATACTAATGATTCACAAACGTATGCTCAC TCAAGCGTTTCATTTACAGGATGTGGCTAAGAAATACGGAAAGAGCGTGGCACAAATCTGTCTGAGGTGG GGAATTCAGAGGAAAACAGCAGTGATTCCAAAATCATCGAAAATTCAGCGATTGAAAGAGAATTTGGAGG TTCTTGAATTCCAGCTGAGCGATGAAGACATGCAGCTCATCTACAGTATCGACAGGAAGTATCGTACCAG TCTACCTTCCAAGACTTGGGGCTTAGACGTGTATGCATAA | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 10 February 2006 - Time: 18:43 Sequenced cultivar: Greensleeves Description: Malus domestica NADP-dependent sorbitol 6-phosphate dehydrogenase (S6PDH) gene, complete cds. View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
210:216 208:224 208:216 208 216 216 216 202:216 |
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