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AT000400-SSR
Primer features
Name Sequence Length Tm  
AT000400-SSR.F CTCCCTTTGCTCCCTCTCTT 20 nt 60.5 °C Primer Stats
AT000400-SSR.R AGGATGTCAGGGTTGTACGG 20 nt 60.5 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 198-232
Number of alleles detected 7
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Fiesta-2 Apple Integrated Map-2
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks extra bands
Sequence
>gi|3088306|gb|AT000400.1|AT000400 AT000400 Apple peel cDNA library Malus x domestica cDNA clone ap189, mRNA sequence CTCCCTTTGCTCCCTCTCTTCGTTTTCTCTACTTTTATTTCGTATCGAAGTAGAACGACGATACNAAATT CAGAGACAGATTCTTTCGAATAANCAAAGCAAAAAAATCAAATAAATAAATCGGAGAATAANGGGAAGAG AGGTTCCACAGCAACAGCAGCAGCAGCAGGNNNCNCNCNTGTGGAGCANTGCTCGCCGTGAATCCGTACA ACCCTGACATCCTTNCCGATCTCGAAAACTACNCAACNNGCAGGTTTCTCTCNCAAACCTACAGTCTCGA TNCAACTCT
User's notes
LucaG (luca.gianfanceschi@unimi.it)
Date 10 February 2006 - Time: 19:18

Sequence

Title: AT000400 Apple peel cDNA library Malus x domestica cDNA clone ap189, mRNA sequence.
Type: mRNA
Length: 289 bp
Organism: Malus x domestica
GenBank Accession: AT000400

Assembly

This sequence was assembled into contig

Library

Name: Apple peel cDNA library
Cultivar: Fuji
Tissue:peel
Description: Vector: Lambda vector UniZap II; initial pfu: 2*106;average insert size:1.3 Kb

PlantGDB link
Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
 		198:216:226
216
216:224
210:224:232
210:224:232
198:210:216:226:232

210:216:232

214:224

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