CH02c02a
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH02c02a.F | CTTCAAGTTCAGCATCAAGACAA | 23 nt | 59.3 °C | ||
CH02c02a.R | TAGGGCACACTTGCTGGTC | 19 nt | 59.5 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 129-176 | ||||
Number of alleles detected | 9 | ||||
Expected heterozygosity | 0.88 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 194 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Discovery-2 Regia x Pingo-2 Regia x Piflora-2 Discovery-2 Fiesta-2 Fiesta x Totem-2 Apple Integrated Map-2 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH02c02a aattgagggtgttagttagggttttaaagatttgttgttggcgggttttattccgaccgattggagttgatgaagcatga tacaaaaaacgaatcttcaagttcagcatcaagacaatactagtagtaaacttagagagagagagagagagaagagagag agagagagagagagaggaggagagagagagagagagagagagagagagagagagtatcaaacatctgtgagattctggag ggatttcaaacttctaaaaccctaatcaggaccagcaagtgtgccctactaat | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
170 148:160 170:172 148:166 n.d. 134:166 129:136 |
Send comments to Luca Gianfranceschi