AF527800-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| AF527800-SSR.F | TGGAAAGGGTTGATTGACCT | 20 nt | 56.4 °C |  |
|
| AF527800-SSR.R | AACAGCGGGTGGTAAATCTC | 20 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 168-194 | ||||
| Number of alleles detected | 5 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-17 Fiesta-17 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean, compl. band(s) | ||||
| Sequence | |||||
| >gi|22023948|gb|AF527800.1| Malus x domestica expansin 3 (EXP03) mRNA, complete cds AATTCGGCACGAGGCTCTTCATTTCCTCACCACTTCTTCATCTTATCAGCATTTCCTCACTGAAAATGGC TTTTTTCGGTATTCTTGTAATTGGGTTTCTCTCACTTGTCTCTTCTGTTAATGGCTACTATGGAGGTTGG TCCAATGCTCATGCAACCTTTTATGGAGGTGGTGATGCTTCCGGCACAATGGGTGGGGCTTGTGGGTATG GGAACCTCTACAGCCAGGGCTATGGGACTAACACAGCAGCTCTAAGCACTGCATTGTTCAACAATGGCTT GACCTGTGGAGCATGCTATCAGATCAGGTGTGTGAATGACCCACAGTGGTGCCTCCCTGGCTCCATCATA GTTACTGCCACAAACTTTTGCCCACCAGGTGGCTGGTGCGACCCTCCCCAGCAACACTTTGATCTCTCTC AGCCTGTCTTCCTCCGCATTGCCCAATACAAAGCTGGGGTTGTCCCAGTGTCCTACAGAAGGGTTAGATG CAGGAGAAGAGGAGGCATAAGGTTCACTGTGAATGGACACTCTTACTTCAACTTAGTCCTTGTGACAAAC GTCGGTGGTGCTGGAGATGTGCAATCTGTGGCCATCAAGGGCTCCAGGACTCGGTGGCAAGCAATGTCAA GAAACTGGGGCCAAAACTGGCAGAGCAATTCTTACCTCAATGGACAAAGCCTCTCTTTTATTGTGACCAC CAGTGATGGCCGCAGATTGGTTTCTTACAATGTTGCCCCTCCGAATTGGTCATTTGGTCAGACATACACT GGGAGACAGTTTCTCTATTAGTTAATTATCTCTCTATGTATGGTTAAAAAAGCTTATTTTACTACTCCAG TTTGTTTAGCTGTTTGTTTAGCTGCATATGTGGTAAAAATTTAAGGGTAAAAGATGAAAAGGGTTTGCTT ATAAGGGTAAAAAAATGGAAAGGGTTGATTGACCTCTTTCATTTTATTATAGATTTGTTAGTTTTTTCAG GTCATGGCTTGGATTATTCTGAAGCCTCATGGCCATATCTGCTAGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAATCAGTTTGCAAGGCAGTTAGGTAGTGGAGATTTACCACCCGCTGTTAGTTCATGAAGTTATGAAT ATTGAATAGAAGAGGTGTTTCTTTTACTACGATTAGCAAATGCTGTAGCAGAAGAGGTCCTACAATCAGC TTGTGTAATGTCATTGTAATAAACTCGGGCTGCTTATTCTGCGCCTTTAATTACATTCTAAGCCGATATT AATCTAAACTACTCTAATTTATGGGATGAAAAAAAAAAAAAAAAAAAAAAA | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 10 February 2006 - Time: 18:49 Title: Malus x domestica expansin 3 (EXP03) mRNA, complete cds. Cultivar: Royal Gala Tissue: over-ripe fruit This sequence was assembled into a contig | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
178:nul 168:184 168:178 168 168 168 168:194 nd |
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