AJ001681-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| AJ001681-SSR.F | CCTGAGGTTATTGACCCAAAA | 21 nt | 57.4 °C |  |
|
| AJ001681-SSR.R | CACTCAGTTGGAAAACCCTACA | 22 nt | 60.3 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 169-195 | ||||
| Number of alleles detected | 6 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Fiesta-17 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean | ||||
| Sequence | |||||
| >gi|3646333|emb|AJ001681.1|MDAJ1681 Malus domestica mRNA for MADS box protein MdMADS8 TTGGAACCTGAGGTTATTGACCCAAAAGAAAAATCAGGATTGGAACCTGAGGTTAAGAAGATAAGCCATC ACTCAAGAGACAGAACTACAAGACAATCAGCTCTAATCTTTCTCTCTCTTCCACTCTCTCTCTCTCTCTC TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTAGGGTTTTCCAACTGAGTGAGTATCTAACTTTC TCTTGTGTTTCACAAATTTGGTTACAGTTTGGTACTGACCTAGAAAAGGAGGATCAGTTTGTACCAAAGA GAGAGAGAAGAACAAGAAAGGGAAAGAAAATATTGTCAAGATCAAAACTTTTGGAGATTAGATACACAAA ATAATATTTTTAGTAACTAAGGACATGGGGAGAGGAAGAGTGGAGCTGAAGAGGATAGAGAACAAGATAA ACAGGCAAGTCACATTTGCAAAGAGAAGAAATGGGCTTCTGAAGAAAGCTTATGAGCTCTCCGTTCTCTG TGATGCTGAGGTTGCTCTCATCATCTTCTCCAACCGTGGCAAGCTCTATGAGTTTTGCAGCAGCTCTAGC ATTCTCAAAACACTAGACAGGTACCAAAAATGCAGTTATGGTGCAGTGGATCAAGTTAATAGACCTGCAA AGGAACTTGAGAGCAGCTATCGTGAGTACATGAAACTCAAAGGTAGATATGAGTCCCTACAACGAACTCA GAGAAACCTTCTTGGTGAGGACTTGGGTCCATTAAACACAAAGGAACTTGAGCAGCTTGAGCGTCAACTT GAGGGCTCCTTGAAGCAAGTTAGGTCCACTAAGACCCAGTATATGCTGGACCAACTTTCTGACCTTCAGA ACAAGGAACAACTGTTAATAGAAGCCAACAGGGATTTGACAATGAAGTTGGATGAAATTAGTTCAAGAAA TCAACTTAGACAATCATGGGAAGGTGGTGACCAGGGTATGGCATATGCAACCCAGCATCACCATGCTCAA TCCCAAGGATTCTTCCAGCCTTTAGATTGCAATCCCACTTTGCAAATGGGGTACTCTGCAGTGGGGTCAG AGCAGATGAGTGCTACAACTAATGCCCAGCAAGTGAACTGTTTCATCCCTGGATGGATGCTTTGAGCATG TGCTGATGAATCTGATCAGTGCCTCTAATCACTCATAAGAAAGCATGCCCAATTATGCTTTTTTTTTCCT GGCAATATTATATAAAATGCATGTAACTTATTTTCACATAAACTCAGCAGTGACTGAATTTCAAGGGGAC CTTTCAAAGGCCTAAAGTCCTTTTAGAACTC | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 10 February 2006 - Time: 18:54 Title: Malus domestica mRNA for MADS box protein MdMADS8. Cultivar: Granny Smith Developmental stage: flower, fruit This sequence was assembled into contig View sequence information at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
169:191 191 189 169:187 169:187 183 191 189 |
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