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AT000174-SSR
Primer features
Name Sequence Length Tm  
AT000174-SSR.F CGGAGGCCGCTATAATTAGG 20 nt 60.5 °C Primer Stats
AT000174-SSR.R CCTGGAAAGAAAGTAAAAGGACA 23 nt 59.3 °C
SSR info
SSR repeat type Compound
Locus type presumed multi-locus
Detected loci
Alleles size range 178-200
Number of alleles detected 6
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-17 Fiesta-17 PI 613988-17 Apple Integrated Map-17
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks extra bands
Sequence
>gi|3088080|gb|AT000174.1|AT000174 AT000174 Apple young fruit cDNA library Malus x domestica cDNA clone af180, mRNA sequence AGGTCCTGCAAGAGACTTGCAACTATATCAGAAACTTACACAGAGAGGTTGACGACCTAAGCGAGCGACT CTCCCAACTGCTCTCTACAATTGATGCTGATAGTCCGGAGGCCGCTATAATTAGGAGCTTGATTACGCAG TAGATGGATCATCAGATCATGATGAGAAACCCTAATATTATATATATATATATATATATATATATATATG TATGTATGTATGTATATGTATATGTGTGTGTATGTGTGTCTAAGAGCATTTTAATTTATATGTTAAGTGT CCTTTTACTTTCTTTCCAGGTTAGAGTACTTAGTACTGTAT
User's notes
LucaG (luca.gianfranceschi@unimi.it)
Date 10 February 2006 - Time: 19:12
Title: AT000174 Apple young fruit cDNA library Malus x domestica cDNA clone af180, mRNA sequence.
Cultivar: Fuji
Developmental stage: Apple young fruit, 7-10 days after anthesis
This sequence was assembled into contig
View Sequence Info at PlantGDB
Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
 		186:194
186:192
178
178:186
178:186
186:188

178:200

178

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