AT000420-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| AT000420-SSR.F | TTGGACCAATTATCTCTGCTATT | 23 nt | 57.5 °C |  |
|
| AT000420-SSR.R | GATGTGGTCAGGGAGAGGAG | 20 nt | 62.5 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 189-209 | ||||
| Number of alleles detected | 5 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-4 Apple Integrated Map-4 M.27 x M.116-4 M.27 x M.116-10 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | extra bands | ||||
| Sequence | |||||
| >gi|3088326|gb|AT000420.1|AT000420 AT000420 Apple peel cDNA library Malus x domestica cDNA clone ap212, mRNA sequence GTTGGACCAATTATCTCTGCTATTAATTTTCTAGAGAGAGAAAGAGGAGAGAGAGAGAGAGAGTGATTTC TACGCATAAATGTTCATGTTTGTACGAATGAGTGAATGAGAACTCCGCCTTCTCTGCTATCTCTCACCAT TGACTTAGCTGTCCTCAACCTCCCCAGTATATCCGATCTCTCTCCTCTCCCTGACCACATCCTCCTCGAT CTCTTCCTGAAAACTTTGAGAGCAGGAAAGCTAAATGAGAAAGTGTTGAAGCTGTTTGTAGCAACTGGCA AGGAAGAAGTCTTTCACTAATCCAGTCACTTATATTCGGCAGACCCTCACCCTGTCTTCTACCAGATGCC TGAAAGTTTGAGATTGGGTGTNGTGGCAGAGTATATACTTGAGACAAGA | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 10 February 2006 - Time: 19:22 View Sequence Info at PlantGDB Sequence Assembly Library | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
201 203:205 201:203 201:205 201 203:209 201:205 201:205 |
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