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AU223486-SSR
Primer features
Name Sequence Length Tm  
AU223486-SSR.F TGACTCCATGGTTTCAGACG 20 nt 58.4 °C Primer Stats
AU223486-SSR.R AGCAATTCCTCCTCCTCCTC 20 nt 60.5 °C
SSR info
SSR repeat type Compound
Locus type single locus
Detected loci
Alleles size range 205-217
Number of alleles detected 3
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 0
Linkage group(s)
Developed by HiDRAS consortium
Other maps Discovery-13 Apple Integrated Map-13
Reference publication Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224
Remarks extra bands
Sequence
>gi|15184510|gb|AU223486.1|AU223486 AU223486 Apple shoot cDNA library Malus x domestica cDNA clone S0016, mRNA sequence GATGGTGAGAGTGATGACTCCATGGTTTCAGACGCCTCCTCCGGCCCAAGCCACCGTGAAGAAGTACTAC TTCCACGTGGGATCGGCGGCAGAAATCGCAGCGGCGGATGGCGGCTCAGGCATGCAGATAGAGATAAGAA AGTAACTTGCAGAAAGGAGAAGAAGAGAGAGGATGAAAGATTAAGGGTCAGAGAAAAGGGGGAGGAGGAG GAGGAGGAATTGCTGCACAAGGCAGATAGTGCTGAAAGTCAGGTGTGAAGTTTGGACTGCAGTTGAGCAG CCAATTAAGGTAATTAAGAAAGATTAATCGGAGGGTTAGAGGAGAATTTAGCTTTCCATTAGTGTGATTA GTATTGAGTTTAAGGTTAATTAAGATGCATATTTGGCGGTGGTTAACAATCAAAGTGTTGTAATTGAGAA AACTGGCACAAACACGAACTAAAGAGGGTGGTGTCCTTGTAATAGATTCAAGTTCAAATCTTACTCCGCT TNAATTAGTTTACTGTA
User's notes
LucaG (luca.gianfranceschi@unimi.it)
Date 10 February 2006 - Time: 19:26

View Sequence Info at PlantGDB

Sequence
Title: AU223486 Apple shoot cDNA library Malus x domestica cDNA clone S0016, mRNA sequence.
Type: mRNA
Length: 507 bp
Organism: Malus x domestica
GenBank Accession: AU223486

Assembly
This sequence was assembled into contig

Library
Name: Apple shoot cDNA library
Cultivar: Fuji
Description: Shoots maintained in vitro culture for 2 years
Allele size and gel image
Cultivar Allele size
Fiesta
Discovery
Gala
Florina
Nova Easygro
TN10-8
Durello di Forli
Prima
Modial Gala
Fuji
 		205
205:217
205:208
205:208
208
205:217

205:208

208

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