AU223548-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| AU223548-SSR.F | ACCACCACTGCAGAGACTCA | 20 nt | 60.5 °C |  |
|
| AU223548-SSR.R | GACGCACCCATTCATCTTTT | 20 nt | 56.4 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 262-278 | ||||
| Number of alleles detected | 4 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Fiesta-10 Apple Integrated Map-10 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | extra bands | ||||
| Sequence | |||||
| >gi|15184572|gb|AU223548.1|AU223548 AU223548 Apple shoot cDNA library Malus x domestica cDNA clone S0279, mRNA sequence ACTTTCCACTGCTTGTNAAACCCAATTATTCAAAGTACCATTTTNNTGCTCTGCACCACCACTGCAGAGA CTCAGAGACTCAGAGACTCAGAGACTCAGATATTCAGAGACCGAGTGAAGTACATCCTCTGCTTCCATCG TCGGTCCAAACTTGGAGTACAAGTACCTCCTCCTCCTCCTCCCTTTTNATNATNTGNATCCTGATCGGAT TTCGGAACAAAAGATGAATGGGTGCGTCCCCGATTTCGAAATGGACGACGATTACTCACTTCCCACTTCC TCTGCTCTCAACCGACCCAG | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 09:51 Sequence Assembly Library
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| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
270:278 270 262:270 262:278 262:270 274 262:270 262:278 |
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