AU223657-SSR
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| AU223657-SSR.F | TTCTCCGTCCCCTTCAACTA | 20 nt | 58.4 °C |  |
|
| AU223657-SSR.R | CACCTTGAGGCCTCTGTAGC | 20 nt | 62.5 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 219-233 | ||||
| Number of alleles detected | 6 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 0 | ||||
| Linkage group(s) | |||||
| Developed by | HiDRAS consortium | ||||
| Other maps | Discovery-3 | ||||
| Reference publication | Silfverberg-Dilworth E, Matasci C L, Van de Weg W E, Van Kaauwen M P W,Walser M, Kodde L P, Soglio V, Gianfranceschi L, Durel C E, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh) genome. Tree Genetics & Genomes 2(4): 202-224 | ||||
| Remarks | clean | ||||
| Sequence | |||||
| >gi|15184681|gb|AU223657.1|AU223657 AU223657 Apple shoot cDNA library Malus x domestica cDNA clone S0159, mRNA sequence ATTAACCTTTGCTGCCCACTCTCCACCTCTCCCCAATCCACTCGCTAGTCGCTACTGCTTTCTCCGTCCC CTTCAACTACTCCGTCTCCATCCTCTCTCTCTCTCTCTCTCTCTATCTCTCTAGAGCTCCGACCCTCTGC GGTCATGGCTACAGTCCTCGACTCCGTCGTCGCTCCCCGATCCTCCGCTCTCCCTTCTTCAGCCGCGCTC TCTCCGATCTCGGCTCCCTCCTCGCCCTCGTCCTTGTCCGCCGGCGCTCGGTTGCCTCGCTACAGAGGCC TCAAGGTGGGATCGGCTCTCCTAACTCGCTCCGCCAGATCGCTGAGTTACGGTCCTGCTTCCGGACGAGC GAGCCGCCGTGGTGGTCTCGTCGTCTGTGAGGCTCAGGACACNGNNACNAGAAGTGCCTGCTGTTGGTGA TGCAACATGGCAGTCACTTGTCCTTGATTCTGAAACCCCTGTTTTGGTTGAATTCTGGGCTCCGTGGTGC GGGCCTTGCGTATGA | |||||
| User's notes | |||||
| LucaG (luca.gianfranceschi@unimi.it) Date 13 February 2006 - Time: 09:54 Sequence Library View Sequence Info at PlantGDB | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Fiesta Discovery Gala Florina Nova Easygro TN10-8 Durello di Forli Prima Modial Gala Fuji |
225 221:225 231 225:231 219:225 231 231 nd |
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