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CH01a07b
Primer features
Name Sequence Length Tm  
CH01a07b.F AACCCATGAAACACAATCCC 20 nt 56.4 °C Primer Stats
CH01a07b.R GGAACGATCCATAGGTGGTG 20 nt 60.5 °C
SSR info
SSR repeat type Perfect
Locus type presumed multi-locus
Detected loci
Alleles size range 206-246
Number of alleles detected 8
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 232
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-3 Fiesta x Totem-15 Fiesta x Totem-16 M.27 x M.116-15 M.27 x M.116-16
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01a07b aattcctatcaacccatgaaacacaatcccagaaatctttctccctctctctctctctct ctctctctctctctctctctctctctctctctttttccagaaatctctagcaaagcaacc atggctatctctctgcaactctccttcccggagcacagacataccacacccacaccccac cgtgacctccaccacagtggcgcacactccggcgagcaccatcaccacctatggatcgtt cctcctccttttctcacctcccgacgagcaccatcaggcaaggggatccatcaaacggtt tgattcaaacccgagcgggttttgggatccggagaaaatggatttttcatttccgacgat ggaggcgcanatcttgggtctgagcctggattgcananagagatctgggcanctgcgaca ccanccttggctgcctttccgaagacggagccacnacgattgtttcgatantatcnaata tatcgcgatattatcncnatattttgacaaaaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking










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