CH01b12
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH01b12.F | CGCATGCTGACATGTTGAAT | 20 nt | 56.4 °C |  |
|
| CH01b12.R | CGGTGAGCCCTCTTATGTGA | 20 nt | 60.5 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | multi-locus | ||||
| Detected loci | |||||
| Alleles size range | 125-178 | ||||
| Number of alleles detected | 9 | ||||
| Expected heterozygosity | n.d. | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 150 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Braeburn-8 Discovery-12 Discovery-13 Fiesta-4 Fiesta-13 Fiesta x Totem-13 Apple Integrated Map-4 Apple Integrated Map-12 Apple Integrated Map-13 M.27 x M.116-4 M.27 x M.116-12 M.27 x M.116-13 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH01b12 ttcgagttgtgccgcatgctgacatgttgaatctctttaaaacttcntcaatgtaacttctttgtgacaaccgtaataat cccttctctctctctctctctctctctctctctctctctctctcttttaatctcgatggngatcacataagagggctcac cgacttggtatcatctaaaaaaatcatactatttgtnggcaataatatgtcatcaacattgagtaccaaaataatgaagt tgctcccactgactttcaagtaaatgcanacatcatangggttttccacanngccatgatccttaacaacacgatgggaa tt | |||||
| User's notes | |||||
| Luca Gianfranceschi (luca.gianfranceschi@unimi.it) Date 7 October 2005 - Time: 16:07 New features have been added to the web pages. Please pay attention to the difference between Locus and Marker type. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
127:128:178 125:127:160:178 126:128:152:nul 126:128 127:128:178 125:127:178 126:127:178 |
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