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CH01c06
Primer features
Name Sequence Length Tm  
CH01c06.F TTCCCCATCATCGATCTCTC 20 nt 58.4 °C Primer Stats
CH01c06.R AAACTGAAGCCATGAGGGC 19 nt 57.3 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 146-188
Number of alleles detected 6
Expected heterozygosity 0.73
PCR annealing temp 60
Sequenced allele size 184
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta-8 Discovery x TN10-8-8 Discovery-8 Fiesta-8 Fiesta x Totem-8 Northern Spy-8 Discovery-8 PI 613988-8 Malling9-8 Robusta5-2 Apple Integrated Map-8 M.27 x M.116-8
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01c06 aattcacatgaggctgatgatctgctgctccaacactaaaaacaaacaatcccattactaacaagagcaatatactcctc cttttccccatcatcgatctctccgcttctcactcacaatcttgcacagagagagagagagagagagagagagagagaga gagagagagagagagagagagagagaggaatgtgtctggtttaagaggaggaagataagaatgtcttgattatataccgt gcgtgcatgccctcatggcttcagttttagtccacgtgtcgaatgagcaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
156:160
160:170
160:172

172:188
160:188
160:188
146:160


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