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CH01d03
Primer features
Name Sequence Length Tm  
CH01d03.F CCACTTGGCAATGACTCCTC 20 nt 60.5 °C Primer Stats
CH01d03.R ACCTTACCGCCAATGTGAAG 20 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type multi-locus
Detected loci
Alleles size range 136-160
Number of alleles detected 5
Expected heterozygosity n.d.
PCR annealing temp 60
Sequenced allele size 155
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Discovery-12 Fiesta x A871-14-12 Discovery-12 Fiesta-4 Fiesta x Totem-4 Royal Gala-12 Malling9-4 Robusta5-4 Apple Integrated Map-4 Apple Integrated Map-12 M.27 x M.116-12
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH01d03 aattgagcacgattgcatggaccnctcccatctcctccacttggcaatgactcctcagcccctacgctgtctcatttatt aanttcacatgcncanaccacctctctctctctctctctctctctctctctctctctctctctgccattctttctttcat ctgtatgantacttcacattggcggtaaggttttaacggcctccaatgattatgtataaggatatanaagtaaaaaaatg atccgatttgtttantaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
138:158
138:136
138:144

138
138:158
158
138


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