CH01g12
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH01g12.F | CCCACCAATCAAAAATCACC | 20 nt | 56.4 °C |  |
|
| CH01g12.R | TGAAGTATGGTGGTGCGTTC | 20 nt | 58.4 °C | ||
| SSR info | |||||
| SSR repeat type | Imperfect | ||||
| Locus type | single locus | ||||
| Detected loci | |||||
| Alleles size range | 112-186 | ||||
| Number of alleles detected | 6 | ||||
| Expected heterozygosity | 0.73 | ||||
| PCR annealing temp | 60 | ||||
| Sequenced allele size | 181 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Discovery x TN10-8-12 Fiesta x Discovery-12 Fiesta x A871-14-12 Discovery-12 Fiesta-12 Fiesta x Totem-12 PI 613988-12 Royal Gala-12 Malling9-12 Robusta5-12 Apple Integrated Map-12 M.27 x M.116-12 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | none | ||||
| Sequence | |||||
| >CH01g12 TTATCAGTGTTGGAAACACTGTTTTTGATAAAGGGATAGATTTTCTAAGTACTTTTCGCT ATGCAAATCTGAAAAACCCTATCCCTTTTCTATTTTAAAGACCATCCCGTGTGCCTTCAA ATCCCTCACAACCAATCAAGTATTTTCCCTCATTTTACTCCCCACCAATCAAAAATCACC CTCACTTCTCTCCCCAATCAGATTTCATTCTCTCTCTATCTCTCTCTCTCTCTCTCTCTC TCTCTCTCTCTTTCTCTCTATCTCTCTCTCTCTCTCTCTTTCTCTCTCTCTCATCTTCTC TCTCTCCTCACTCGAACTCTTGAACGCACCACCATACTTCATAGATCGAGCTTAAAAACA CCACCATCACACTCATCTTGAGCTCACGAAACCCAACCATACCAATTGGAATTCCAATTG TGGCAANTGNGTTACACACGCTACCGCTCTGGAATTCTTTTATTTTAGTTTTAGGTCTCT CCCCTATAAAATCAAACC | |||||
| User's notes | |||||
| THERE ARE NO USER'S NOTES. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size |  | |||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
112:152 112:156 114:152 112:152 112:186 112:184 112 |
||||
Send comments to Luca Gianfranceschi
