CH01h11
| Primer features | |||||
| Name | Sequence | Length | Tm | ||
| CH01h11.F | AgcCATCTCTCAGTTTGAAG | 20 nt | 52.3 °C |  |
|
| CH01h11.R | TTTATAGGCAGAGACCTTCAG | 21 nt | 57.4 °C | ||
| SSR info | |||||
| SSR repeat type | Perfect | ||||
| Locus type | presumed single locus | ||||
| Detected loci | |||||
| Alleles size range | 160-186 | ||||
| Number of alleles detected | 4 | ||||
| Expected heterozygosity | 0.52 | ||||
| PCR annealing temp | 55 | ||||
| Sequenced allele size | 170 | ||||
| Linkage group(s) | |||||
| Developed by | ETH Zuerich | ||||
| Other maps | Fiesta x Totem-13 M.27 x M.116-13 | ||||
| Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
| Remarks | Error in old primer sequence. Use the new one. | ||||
| Sequence | |||||
| >CH01h11 aattgaataacagtgagtaatctaagaatgggaaggatataaaatatagagatcattacctccatacataacatgttatt ggttaacaccacaagccatctctcagtttgaagcttcttattcatttctcttatacatatataaacttcttgctacttct actctctctctctctctctctctctctctctctctctctctctctctcggtttcttctttcttgtctccctctcgtttca ntctgaaggtctctgcctataaatacatagcttcagttggttaggtnctccctctctctctctctctctttcggtttctt ctttcttgtctccctctcgtttcaatctaaaggtctctgcctataaatacatancttcactcggttgggtngtangataa tgacaagcaagttgccactaactaaaaataagagaatt | |||||
| User's notes | |||||
| Luca Gianfranceschi (luca.gianfranceschi@unimi.it) Date 13 September 2005 - Time: 15:08 Forward primer sequence was wrong. Please use the new one. | |||||
| Allele size and gel image | |||||
| Cultivar | Allele size | ||||
|
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
172 172:186 160 n.d. 172 172 172 |
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