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CH02a03
Primer features
Name Sequence Length Tm  
CH02a03.F AGAAGTTTTCACGGGTGCC 19 nt 57.3 °C Primer Stats
CH02a03.R TGGAGACATGCAGAATGGAG 20 nt 58.4 °C
SSR info
SSR repeat type Imperfect
Locus type single locus
Detected loci
Alleles size range 144-170
Number of alleles detected 7
Expected heterozygosity 0.77
PCR annealing temp 60
Sequenced allele size 148
Linkage group(s)
Developed by ETH Zuerich
Other maps Discovery-16 Fiesta-16 Fiesta x Totem-16 PI 613988-16 Robusta5-16 Apple Integrated Map-16
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02a03 aatttgcaagatattattgctattttggggatggacgagctcagtgaagatgataagttgactgtagcccgtgctcgtaa gatttcacggttcttgagtcagccgttccacgttgcagaagttttcacgggtgcccctgggaagtacgtggagttgaaag aaagcattacctccttccaggtaccccctctccctcctctctctctctctctctctctctctctctctctctctctctct caatctccattctgcatgtctccatatatttcacactgaagttctttctattatcatctcagttacctgcttacaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
150:170
150:154
148:150

148:154
150:154
150:154
150:156


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