CH02b10
Primer features | |||||
Name | Sequence | Length | Tm | ||
CH02b10.F | CAAGGAAATCATCAAAGATTCAAG | 24 nt | 58.4 °C | ||
CH02b10.R | CAAGTGGCTTCGGATAGTTG | 20 nt | 58.4 °C | ||
SSR info | |||||
SSR repeat type | Perfect | ||||
Locus type | single locus | ||||
Detected loci | |||||
Alleles size range | 121-159 | ||||
Number of alleles detected | 8 | ||||
Expected heterozygosity | 0.86 | ||||
PCR annealing temp | 60 | ||||
Sequenced allele size | 134 | ||||
Linkage group(s) | |||||
Developed by | ETH Zuerich | ||||
Other maps | Regia x Pingo-2 Regia x Piflora-2 Fiesta-2 Fiesta x Totem-2 Malling9-2 Robusta5-2 Apple Integrated Map-2 | ||||
Reference publication | Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241 | ||||
Remarks | none | ||||
Sequence | |||||
>CH02b10 aattcccaaacttctgctataaaaacgttatttggggtaataaatctacaagcaaggnaaatcatcaaagattcaagatt gaaggcggagagaaacaggcgccattatttgccttgctctctctctctctctctctctctctctctctctctctcacttt ctgtccccaactatccgaagccacttgcaacgtctcctttttcttctttcaaataagaaaaaagttaaataacggactcg gtgtaaatataatacaaatatnggtcaacacaccctcataaatccctattcacctactccccttgttcttcctcaagcgg ctttgatttcacggtaagtcctctctctctctctctctctctggaatt | |||||
User's notes | |||||
THERE ARE NO USER'S NOTES. | |||||
Allele size and gel image | |||||
Cultivar | Allele size | ||||
Prima Fiesta Discovery Durello di Forlí TN10-8 Florina Nova Easygro Starking |
123:127 121:127 131:131 123:127 133:135 135:159 133:159 |
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