Select a marker

CH02b10
Primer features
Name Sequence Length Tm  
CH02b10.F CAAGGAAATCATCAAAGATTCAAG 24 nt 58.4 °C Primer Stats
CH02b10.R CAAGTGGCTTCGGATAGTTG 20 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type single locus
Detected loci
Alleles size range 121-159
Number of alleles detected 8
Expected heterozygosity 0.86
PCR annealing temp 60
Sequenced allele size 134
Linkage group(s)
Developed by ETH Zuerich
Other maps Regia x Pingo-2 Regia x Piflora-2 Fiesta-2 Fiesta x Totem-2 Malling9-2 Robusta5-2 Apple Integrated Map-2
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02b10 aattcccaaacttctgctataaaaacgttatttggggtaataaatctacaagcaaggnaaatcatcaaagattcaagatt gaaggcggagagaaacaggcgccattatttgccttgctctctctctctctctctctctctctctctctctctctcacttt ctgtccccaactatccgaagccacttgcaacgtctcctttttcttctttcaaataagaaaaaagttaaataacggactcg gtgtaaatataatacaaatatnggtcaacacaccctcataaatccctattcacctactccccttgttcttcctcaagcgg ctttgatttcacggtaagtcctctctctctctctctctctctggaatt
User's notes
THERE ARE NO USER'S NOTES.
Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
123:127
121:127
131:131

123:127
133:135
135:159
133:159


admin Send comments to Luca Gianfranceschi