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CH02b11
Primer features
Name Sequence Length Tm  
CH02b11.F ACCTTACCTGGTAGAGAGAGA 21 nt 59.4 °C Primer Stats
CH02b11.R CACAACGCAGTTCGATCACT 20 nt 58.4 °C
SSR info
SSR repeat type Perfect
Locus type presumed single locus
Detected loci
Alleles size range 114-158
Number of alleles detected 7
Expected heterozygosity 0.84
PCR annealing temp 55
Sequenced allele size 102
Linkage group(s)
Developed by ETH Zuerich
Other maps Fiesta x Totem-7 M.27 x M.116-7
Reference publication Liebhard, R. Gianfranceschi, L. Koller, B. Ryder, C. D. Tarchini, R. Weg, E. van de Gessler, C. (2002) Development and characterization of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding 10(4): 217-241
Remarks none
Sequence
>CH02b11 aattaccttacctggtagagagagagagagagagagagagagagagagagagagagagagagagagagagagagagagag agaggaagtgatcgaactgcgttgtgttccgccaaacggattgtttgtggtgttgtcactccccttcacttcgcctagtt gtttttgtgtcctagagagagagggcacttttcgatagggtgtacatggtttggtctagtgtgatttgtagtaatattct tttaagttagaggttttagattcgattctcgtcaaaggcgaatt
User's notes
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Allele size and gel image
Cultivar Allele size
Prima
Fiesta
Discovery
Durello di Forlí
TN10-8
Florina
Nova Easygro
Starking
116:122
114:158
116:118

116:156
156:158
118:122
114:156


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